Review



sucrose gradient fractions  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Thermo Fisher sucrose gradient fractions
    Sucrose Gradient Fractions, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sucrose+gradient+fraction/Sucrose/bio_rxiv__64898__2026__03__11__710933-133-5-11
    Average 99 stars, based on 1 article reviews
    sucrose gradient fractions - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Isolation:

    Article Title: Ribosomal Incorporation of Short RNAs from Mitochondrial Genome Recombination Sites in Toxoplasma gondii
    Article Snippet: .. RNA was isolated from total organelle enriched and sucrose gradient fraction samples using TRIzol® Reagent (Invitrogen), followed by the MonarchTM Total RNA Miniprep Kit (New England BioLabs) applying the manufacturer’s protocol for TRIzol® extracted samples. .. Two hundred nanograms of RNA was used to generate sequencing libraries using the NEBNext ® Multiplex Small RNA Library Prep Set for Illumina ® Kit (New England BioLabs) according to the manufacturer’s instructions.

    Article Title: Small RNAs from mitochondrial genome recombination sites are incorporated into T. gondii mitoribosomes
    Article Snippet: .. RNA was isolated from total organelle enriched and sucrose gradient fraction samples using TRIzol Reagent (Invitrogen), followed by the Monarch Total RNA Miniprep Kit (New England BioLabs) applying the manufacturer’s protocol for TRIzol extracted samples. .. Two hundred nanograms of RNA was used to generate sequencing libraries using the NEBNext Multiplex Small RNA Library Prep Set for Illumina Kit (New England BioLabs) according to the manufacturer’s instructions.

    Reverse Transcription:

    Article Title: The Host DHX29 RNA Helicase Regulates HCMV Immediate Early Protein Synthesis
    Article Snippet: Briefly, RNA was extracted from 200 μL aliquots of individual sucrose gradient fractions by using Trizol (Sigma) followed by Turbo DNase (Thermo Fisher) treatment. .. An equal volume of DNase treated RNA from each sucrose gradient fraction was used to prepare cDNA using the High Capacity cDNA Reverse Transcription Kit (Thermo Fisher) according to the manufacturer’s directions and the cDNAs were diluted 1:1 with deionized water prior to PCR. .. Primers for the following mRNAs were used: IE1 (CAAGTGACCGAGGATTGCAA, CACCATGTCCACTCGAACCTT), IE2 (TGACCGAGGATTGCAACGA, CGGCATGATTGACAGCCTG), β-Actin (GACCCAGATCATGTTTGAGACC, GTCACCGGAGTCCATCACGA), eIF4G (GCCATTTCAGAGCCCAACTTCTC, CGGAAGTTCACAGTCACTGTTGG), HSP90 (AGATTCCACTAACCGACGCC, CCGCACTCGCTCCACAAA) and RACK1 (TGGGATCTCACAACGGGCACCA, CCGGTTGTCAGAGGAGAAGGCCA). qPCR was performed using SYR Select Master Mix (Thermo Fisher) and 40 cycles (45 seconds at 60°C followed by 15 seconds at 95°C) in a CFX96 thermal cycler (Biorad).

    Polymerase Chain Reaction:

    Article Title: The Host DHX29 RNA Helicase Regulates HCMV Immediate Early Protein Synthesis
    Article Snippet: Briefly, RNA was extracted from 200 μL aliquots of individual sucrose gradient fractions by using Trizol (Sigma) followed by Turbo DNase (Thermo Fisher) treatment. .. An equal volume of DNase treated RNA from each sucrose gradient fraction was used to prepare cDNA using the High Capacity cDNA Reverse Transcription Kit (Thermo Fisher) according to the manufacturer’s directions and the cDNAs were diluted 1:1 with deionized water prior to PCR. .. Primers for the following mRNAs were used: IE1 (CAAGTGACCGAGGATTGCAA, CACCATGTCCACTCGAACCTT), IE2 (TGACCGAGGATTGCAACGA, CGGCATGATTGACAGCCTG), β-Actin (GACCCAGATCATGTTTGAGACC, GTCACCGGAGTCCATCACGA), eIF4G (GCCATTTCAGAGCCCAACTTCTC, CGGAAGTTCACAGTCACTGTTGG), HSP90 (AGATTCCACTAACCGACGCC, CCGCACTCGCTCCACAAA) and RACK1 (TGGGATCTCACAACGGGCACCA, CCGGTTGTCAGAGGAGAAGGCCA). qPCR was performed using SYR Select Master Mix (Thermo Fisher) and 40 cycles (45 seconds at 60°C followed by 15 seconds at 95°C) in a CFX96 thermal cycler (Biorad).

    RNA Extraction:

    Article Title: IGF-1R inhibition induces MEK phosphorylation to promote survival in colon carcinomas.
    Article Snippet: .. RNA extraction and RT-qPCR Total RNA was extracted from each sucrose gradient fraction using Trizol reagent (Invitrogen). .. The first strand of cDNA was synthesized from each fraction using the Superscript First-Strand Synthesis System (ThermoFisher).

    Quantitative RT-PCR:

    Article Title: IGF-1R inhibition induces MEK phosphorylation to promote survival in colon carcinomas.
    Article Snippet: .. RNA extraction and RT-qPCR Total RNA was extracted from each sucrose gradient fraction using Trizol reagent (Invitrogen). .. The first strand of cDNA was synthesized from each fraction using the Superscript First-Strand Synthesis System (ThermoFisher).

    Mass Spectrometry:

    Article Title: Bottom-up structural proteomics: cryoEM of protein complexes enriched from the cellular milieu
    Article Snippet: .. This yielded the following degenerate sequences, which were then used for searching: Using this set of query sequences, we test the ability of cryoID to correctly identify the corresponding protein, from a candidate pool consisting of the 773 proteins identified in this sucrose gradient fraction by mass spectrometry. cryoID correctly identified the corresponding protein for the query set generated from the Unknown Protein 1 cryoEM map, making a unique protein ID of M18 aspartyl aminopeptidase from P. falciparum (Q8I2J3) ( ). ..

    Generated:

    Article Title: Bottom-up structural proteomics: cryoEM of protein complexes enriched from the cellular milieu
    Article Snippet: .. This yielded the following degenerate sequences, which were then used for searching: Using this set of query sequences, we test the ability of cryoID to correctly identify the corresponding protein, from a candidate pool consisting of the 773 proteins identified in this sucrose gradient fraction by mass spectrometry. cryoID correctly identified the corresponding protein for the query set generated from the Unknown Protein 1 cryoEM map, making a unique protein ID of M18 aspartyl aminopeptidase from P. falciparum (Q8I2J3) ( ). ..



    Similar Products

    99
    Thermo Fisher sucrose gradient fractions
    Sucrose Gradient Fractions, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sucrose+gradient+fraction/Sucrose/bio_rxiv__64898__2026__03__11__710933-133-5-11
    Average 99 stars, based on 1 article reviews
    sucrose gradient fractions - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher sucrose gradient fractions with trizol
    Sucrose Gradient Fractions With Trizol, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sucrose+gradient+fraction/Sucrose/pmc12539630-53-8-13
    Average 99 stars, based on 1 article reviews
    sucrose gradient fractions with trizol - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    97
    Bio-Rad sucrose gradient fraction
    Sucrose Gradient Fraction, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sucrose+gradient+fraction/SDS+(Sodium+Dodecyl+Sulfate)/pmc12092007-704-4-28
    Average 97 stars, based on 1 article reviews
    sucrose gradient fraction - by Bioz Stars, 2026-10
    97/100 stars
      Buy from Supplier

    99
    Thermo Fisher sucrose gradient fraction
    Sucrose Gradient Fraction, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sucrose+gradient+fraction/Sucrose/bio_rxiv__2025__05__08__652939-246-4-34
    Average 99 stars, based on 1 article reviews
    sucrose gradient fraction - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    Image Search Results